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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
15/01/2020 |
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Data da última atualização: |
16/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIRA, S. M.; DIONISIO, A. P.; HOLANDA, M. O.; MARQUES, C. G.; SILVA, G. S. DA; CORREA, L. C.; SANTOS, G. B. M.; ABREU, F. A. P. de; MAGALHÃES, F. E. A.; REBOUÇAS, E. DE L.; GUEDES, J. A. C.; OLIVEIRA, D. F. DE; OLIVEIRA, D. F. DE; GUEDES, M. I. F.; ZOCOLO, G. J. |
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Afiliação: |
SANDRA MACHADO LIRA, Department of Nutrition, State University of Ceara, Fortaleza, CE, Brazil; ANA PAULA DIONISIO, CNPAT; MARCELO OLIVEIRA HOLANDA, Department of Nutrition, State University of Ceara, Fortaleza, CE, Brazil; CHAYANE GOMES MARQUES, Department of Nutrition, State University of Ceara, Fortaleza, CE, Brazil; GISELE SILVESTRE DA SILVA, Embrapa Agroindústria Tropical, Dra Sara Mesquita Street, Fortaleza, CE, Brazil; LIA COÊLHO CORREA, Department of Nutrition, State University of Ceara, Fortaleza, CE, Brazil; GLAUBER BATISTA MOREIRA SANTOS, Federal University of Ceara, Department of Food Technology; FERNANDO ANTONIO PINTO DE ABREU, CNPAT; FRANCISCO ERNANI ALVES MAGALHÃES, State University of Ceara, Department of Nutrition; EMANUELA DE LIMA REBOUÇAS, State University of Ceara, Department of Nutrition; JHONYSON ARRUDA CARVALHO GUEDES, Embrapa Agroindústria Tropical; DANIELLE FERREIRA DE OLIVEIRA, State University of Ceara, Department of Nutrition; OLIVEIRA, DANIELLE FERREIRA DE, State University of Ceara, Department of Nutrition; MARIA IZABEL FLORINDO GUEDES, State University of Ceara, Department of Nutrition; GUILHERME JULIAO ZOCOLO, CNPAT. |
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Título: |
Metabolic profile of pitaya (Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose) by UPLC-QTOF-MSE and assessment of its toxicity and anxiolytic-like effect in adult zebrafish |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Food Research International, Amsterdam, v. 127, artigo 108701, 10 p., Jan. 2020 |
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DOI: |
https://doi.org/10.1016/j.foodres.2019.108701 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Dragon fruit; Frutas exóticas nativas. |
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Thesagro: |
Pitaya. |
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Thesaurus Nal: |
Anxiety; Bioactive compounds; Hylocereus; Secondary metabolites. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01230naa a2200373 a 4500
001 2118827
005 2020-06-16
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.foodres.2019.108701$2DOI
100 1 $aLIRA, S. M.
245 $aMetabolic profile of pitaya (Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose) by UPLC-QTOF-MSE and assessment of its toxicity and anxiolytic-like effect in adult zebrafish$h[electronic resource]
260 $c2020
650 $aAnxiety
650 $aBioactive compounds
650 $aHylocereus
650 $aSecondary metabolites
650 $aPitaya
653 $aDragon fruit
653 $aFrutas exóticas nativas
700 1 $aDIONISIO, A. P.
700 1 $aHOLANDA, M. O.
700 1 $aMARQUES, C. G.
700 1 $aSILVA, G. S. DA
700 1 $aCORREA, L. C.
700 1 $aSANTOS, G. B. M.
700 1 $aABREU, F. A. P. de
700 1 $aMAGALHÃES, F. E. A.
700 1 $aREBOUÇAS, E. DE L.
700 1 $aGUEDES, J. A. C.
700 1 $aOLIVEIRA, D. F. DE
700 1 $aOLIVEIRA, D. F. DE
700 1 $aGUEDES, M. I. F.
700 1 $aZOCOLO, G. J.
773 $tFood Research International, Amsterdam$gv. 127, artigo 108701, 10 p., Jan. 2020
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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Classificação |
Cutter |
Registro |
Volume |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
09/01/2023 |
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Data da última atualização: |
09/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
CASTRO, I. S. L.; BARREIROS, P. R. R. M.; MENDES, T. A. de O.; FLOREZ, J. C.; SILVA, E. M. de A.; PORTO, B. N.; ZAMBOLIM, L.; CAIXETA, E. T. |
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Afiliação: |
ISABEL SAMILA LIMA CASTRO, UNIVERSIDADE FEDERAL DE VIÇOSA; PEDRO RICARDO ROSSI MARQUES BARREIROS, UNIVERSIDADE FEDERAL DE VIÇOSA; TIAGO ANTÔNIO DE OLIVEIRA MENDES, UNIVERSIDADE FEDERAL DE VIÇOSA; JUAN CARLOS FLOREZ, UNIVERSIDADE FEDERAL DE VIÇOSA; EDSON MARIO DE ANDRADE SILVA, UNIVERSIDADE FEDERAL DE VIÇOSA; BRENDA NEVES PORTO, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa. |
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Título: |
Gene expression and interactome analysis of candidate effectors associated with pre- and post-haustorial hemileia vastatrix-coffee interaction. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Biotechnology and Biomedicine, v. 5, n. 4, p. 198-213, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
The present study sought to analyze the putative secreted proteins of Hemileia vastatrix, with potential to function as effector proteins. The H. vastatrix secretome was subjected to functional categorization and the greatest similarities were observed between species of the genus Puccinia sp (398), and Melampsora larici-populina (82). Based on the secretome, 415 Gene Ontology terms were extracted. The putative secretome was also compared to the high-throughput transcriptome of coffee-H. vastatrix interactions. By the transcriptome comparison data and the results of functional annotation and characteristics associated with effector proteins, 15 genes were selected and analyzed using RT-qPCR during compatible and incompatible coffee-H. vastatrix interactions. The expression patterns suggested that the EHv33-18 and EHv33-25 candidate effector may be responsible for faster communication between pathogen and the host during incompatible interaction. Other six candidate are involved in the biotrophic stage of infection, which is characterized by an increase in the expression of effectors, and in enzymes involved in secondary metabolism. Phylogenetic analysis suggest that these eight genes follow evolutionary mechanisms exclusive to the coffee-H. vastatrix interaction, making them important targets in studies aimed at obtaining durable resistance to this disease. Interactomic network made between coffee proteins and H. vastatrix proteins was obtained for the first time and revealed a wide network of interactions between effector EHv33-19 and coffee proteins. The obtained results suggest that there may be communication between the pathogen and the host in the early stage of infection during the urediniospores germination phase. This indicated pre-haustorial resistance complementary to post-haustorial resistance. MenosThe present study sought to analyze the putative secreted proteins of Hemileia vastatrix, with potential to function as effector proteins. The H. vastatrix secretome was subjected to functional categorization and the greatest similarities were observed between species of the genus Puccinia sp (398), and Melampsora larici-populina (82). Based on the secretome, 415 Gene Ontology terms were extracted. The putative secretome was also compared to the high-throughput transcriptome of coffee-H. vastatrix interactions. By the transcriptome comparison data and the results of functional annotation and characteristics associated with effector proteins, 15 genes were selected and analyzed using RT-qPCR during compatible and incompatible coffee-H. vastatrix interactions. The expression patterns suggested that the EHv33-18 and EHv33-25 candidate effector may be responsible for faster communication between pathogen and the host during incompatible interaction. Other six candidate are involved in the biotrophic stage of infection, which is characterized by an increase in the expression of effectors, and in enzymes involved in secondary metabolism. Phylogenetic analysis suggest that these eight genes follow evolutionary mechanisms exclusive to the coffee-H. vastatrix interaction, making them important targets in studies aimed at obtaining durable resistance to this disease. Interactomic network made between coffee proteins and H. vastatrix proteins was obtained for the first time and reveale... Mostrar Tudo |
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Thesaurus NAL: |
Gene interaction; Hemileia; Phylogeny; Plant pathogens. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150799/1/gene-expression-and-interactome.pdf
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Marc: |
LEADER 02645naa a2200253 a 4500
001 2150799
005 2023-01-09
008 2022 bl uuuu u00u1 u #d
100 1 $aCASTRO, I. S. L.
245 $aGene expression and interactome analysis of candidate effectors associated with pre- and post-haustorial hemileia vastatrix-coffee interaction.$h[electronic resource]
260 $c2022
520 $aThe present study sought to analyze the putative secreted proteins of Hemileia vastatrix, with potential to function as effector proteins. The H. vastatrix secretome was subjected to functional categorization and the greatest similarities were observed between species of the genus Puccinia sp (398), and Melampsora larici-populina (82). Based on the secretome, 415 Gene Ontology terms were extracted. The putative secretome was also compared to the high-throughput transcriptome of coffee-H. vastatrix interactions. By the transcriptome comparison data and the results of functional annotation and characteristics associated with effector proteins, 15 genes were selected and analyzed using RT-qPCR during compatible and incompatible coffee-H. vastatrix interactions. The expression patterns suggested that the EHv33-18 and EHv33-25 candidate effector may be responsible for faster communication between pathogen and the host during incompatible interaction. Other six candidate are involved in the biotrophic stage of infection, which is characterized by an increase in the expression of effectors, and in enzymes involved in secondary metabolism. Phylogenetic analysis suggest that these eight genes follow evolutionary mechanisms exclusive to the coffee-H. vastatrix interaction, making them important targets in studies aimed at obtaining durable resistance to this disease. Interactomic network made between coffee proteins and H. vastatrix proteins was obtained for the first time and revealed a wide network of interactions between effector EHv33-19 and coffee proteins. The obtained results suggest that there may be communication between the pathogen and the host in the early stage of infection during the urediniospores germination phase. This indicated pre-haustorial resistance complementary to post-haustorial resistance.
650 $aGene interaction
650 $aHemileia
650 $aPhylogeny
650 $aPlant pathogens
700 1 $aBARREIROS, P. R. R. M.
700 1 $aMENDES, T. A. de O.
700 1 $aFLOREZ, J. C.
700 1 $aSILVA, E. M. de A.
700 1 $aPORTO, B. N.
700 1 $aZAMBOLIM, L.
700 1 $aCAIXETA, E. T.
773 $tJournal of Biotechnology and Biomedicine$gv. 5, n. 4, p. 198-213, 2022.
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Registro original: |
Embrapa Café (CNPCa) |
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